Effects of suberoylanilide hydroxamic acidon proliferation and apoptosis of human hepatic stellate cells
- VernacularTitle:辛二酰苯胺异羟肟酸对人肝星状细胞增殖和凋亡的影响
- Author:
Xing LIU
;
Tian TIAN
;
Wei ZHAN
;
Lei YU
;
Bing HAN
;
Rujia XIE
;
Xinhua LUO
;
Qin YANG
- Keywords:
hepatic stellate cells;
suberoylanilide hydroxamic acid;
histone modifications;
liver fibrosis
- From:Basic & Clinical Medicine
2017;37(4):468-472
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the effects of histone deacetylase inhibitor suberoylanilide hydroxamic acid(SAHA) on the cell proliferation and apoptosis of the human hepatic stellate cell line LX-2.The possible underlying mechanisms were also investigated.Methods The LX-2 cells were treated with SAHA in vitro.The morphology of LX-2 cells in different concentrations groups was observed by inverted microscope;the proliferation of LX-2 cells was measured by MTT assay;the Annexin V-FITC and PI staining was used to detect the apoptosis of LX-2 cells by flow cytometry and fluorescence microscope;the expression of α-SMA,collagen Ⅰ,acH3K9,acH3K14 and acH3K18 were detected by Western blot.Results The morphology change of LX-2 cells showed that SAHA inhibited the proliferation rate of LX-2 cells and in a dose dependent manner(P<0.05).The LX-2 cells were sensitive to SAHA along with time increasing,and in a time-dependent manner(P<0.05).Western blot showed that the expression levels of α-SMA and collagen-Ⅰ were significantly lower(P<0.05),on the contrary,the acetylation levels of acH3K9,acH3K14 and acH3K18 were significantly higher (P<0.05).Conclusions The increased acetylation of the histone acH3K9,acH3K14,acH3K18 and the lower expressed α-SMA and collagen-Ⅰ in LX-2 cells may be one of the mechanisms of SAHA.
