Detection p16INK4a protein in cervical tissues with time-resolved fluoroimmunoassay
10.13315/j.cnki.cjcep.2017.01.005
- VernacularTitle:应用时间分辨法检测石蜡包埋子宫颈组织p16INK4a蛋白
- Author:
Li DING
;
Xianjin ZOU
;
Wanting FAN
;
Jie ZHENG
;
Qiong CHEN
- Keywords:
cervical tissues;
p16INK4a;
immunohistochemistry;
time-resolved fluoroimmunoassay
- From:
Chinese Journal of Clinical and Experimental Pathology
2017;33(1):17-21
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To establish a new method for detecting p16INK4a in cervical tissues with time-resolved fluoroimmunoassay (TRFIA).Methods 126 cases of paraffin imbedding tissues of cervix were selected for immunohistochemistry (IHC) of EnVision two-step and TRFIA.Results There were 20 cases of no intraepithelial lesion or malignancy,24 cases of low-grade squamous intraepithelial lesion (LSIL),53 cases of high-grade squamous intraepithelial lesion (HSIL) and 29 cases of squamous cell carcinoma (SCC).In the groups of no intraepithelial lesion or malignancy,LSIL,HSIL and SCC,p161NK4a positive was seen in 1,19,53 and 28,respectively.TRFIA test results displayed p16INK4a positive in 3,17,50 and 27 cases,respectively.Positive of p16 using by TRFIA in no intraepithelial lesion or malignancy,LSIL,above HSIL was 15.00％,70.83％ and 93.90％,respectively (P ＜ 0.01).Conclusion TRFIA is suitable for detecting of p16INK4a protein and demand low detection equipment,p16INK4a expression detected by TRFIA may helpful for large scale detection in various clinical institution.
