Determination of Caffeine in Urea by Bubble-in-drop Single Drop Microextraction and Gas Chromatography-Mass Spectrometry
10.11895/j.issn.0253-3820.160862
- VernacularTitle:气泡微萃取-气相色谱-质谱法测定尿中咖啡因
- Author:
Aiying SONG
;
Lihong WANG
;
Yanxia ZHANG
;
Enlin SHI
;
Junling ZENG
- Keywords:
Bubble-in-drop single drop microextraction;
Caffeine;
Gas chromatography-mass spectrometry;
Urea
- From:Chinese Journal of Analytical Chemistry
2017;45(5):707-712
- CountryChina
- Language:Chinese
-
Abstract:
A method for the determination of caffeine in urea was developed based on bubble-in-drop single drop microextraction (BID-SDME) followed by gas chromatography/mass spectrometry (GC-MS).Under the optimum conditions including chloroform as extraction solvent, an exposure volume of 1 μL, a bubble volume of 1.6 μL, stirring for 5 min at 300 r/min, 15% (m/V) NaCl, and a distance of 1 cm between bubble and stirring bar, the detection limit of this method was as low as 0.003 mg/L and the linear range was from 0.005 mg/L to 10 mg/L with correlation coefficient of 0.982.The recoveries of caffeine were from 89.2% to 107.5% at different spiked levels in human urine and the relative standard deviation (RSD, n=6) was less than 8%.
