Analysis of Poria cocos Mycelium Browning by Transcriptome Sequencing

VernacularTitle:茯苓菌落褐变的转录组测序分析
Author: Danfeng CAI ; Zhixin CAI ; Meiyuan CHEN ; Yanquan LIN
Keywords: Poria cocos; browning; transcriptome sequencing
From: Journal of Guangzhou University of Traditional Chinese Medicine 2017;34(2):245-249
CountryChina
Language:Chinese
Abstract: Objective To study the browning-related genes of Poria cocos mycelia,so as to provide a basis for their further verification and to discuss the browning molecular mechanism of Poria cocos.Methods Four samples of normal and brown Poria cocos mycelia were analyzed by transcriptome sequencing.After aligned with the reference genome sequence of Poria cocos,the expression levels of the genes in different samples were analyzed.Results A total of 12 383 transcripts were identified.Among them,1 017 genes were firstly found,and 260 genes were functionally annotated.Based on the alignment results,336 common differentially-expressed genes overlapped between the normal mycelia and 3 browned mycelia were obtained,and part of them were evaluated.Conclusion Transcriptome sequencing results showed that plenty of differentially-expressed genes were found in brown Poria cocos mycelia,and the difference of the expression levels of some genes was up to several hundred folds or even thousands of folds,whose related-functions were worth of further analysis.