Optimization of the Extraction Technology of Chlorogenic Acid in Prunus armeniaca Flos and Comparison of Its Contents from Different Origins and Varieties
10.6039/j.issn.1001-0408.2017.07.32
- VernacularTitle:杏花中绿原酸提取工艺优化及不同产地和品种杏花中绿原酸的含量比较
- Author:
Huiyu LUO
;
Weiwei YAN
;
Peng XU
;
Chunping ZENG
- Keywords:
Prunus armeniaca flos;
Chlorogenic acid;
Orthogonal test;
Extraction technology;
Optimization;
Origin;
Variety
- From:
China Pharmacy
2017;28(7):975-978
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To optimize the extraction technology of chlorogenic acid in Prunus armeniaca flos,and compare the contents in P. armeniaca flos from different origins and varieties. METHODS:HPLC was conducted to determine the content of chlorogenic acid in P. armeniaca flos;using the volume fraction of ethanol,the ratio of material to liquid,ultrasonic extraction times and time as factor,the content of chlorogenic acid as index,single factor and orthogonal test were designed to optimize the extraction technology,and verification tests were carried out. The optimized extraction technology was used to extract and compare the contents of chlorogenic acid in Armeniaca sibirica from 7 origins of P. armeniaca flos and 3 origins of Armeniaca sibirica flos. RESULTS:The optimized extraction technology was extracting twice with 12-fold 75% ethanol,30 min each time. Under the con-ditions,the content of chlorogenic acid can reach 77.38 mg/g(RSD=0.58%,n=3);the contents of chlorogenic acid in A. sibiri-ca flos and P. armeniaca flos were 77.38-83.33 mg/g and 63.12-70.22 mg/g,respectively. CONCLUSIONS:The established extrac-tion technology is reasonable,stable and feasible. The contents of chlorogenic acid in A. sibirica are higher than that in P. armenia-ca flos;the contents have no obvious differences in the same variety of A. sibirica from different origins.
