Effects of JAK-STAT signaling pathway, IL-1βand IL-6 on injury of PC12 cells with X-ray irradiation

VernacularTitle:JAK－STAT信号通路、IL－1β和IL－6在X射线辐照诱导PC12细胞损伤中的调控作用
Author: Yu JING ; Zhang YI ; Diao BO
Keywords: X-ray; PC12 cells; JAK-STAT signaling pathway; IL-1β; IL-6
From: Chinese Journal of Pathophysiology 2017;33(1):174-178
CountryChina
Language:Chinese
Abstract: AIM:To investigate the role of JAK-STAT pathway , IL-1βand IL-6 in the PC12 cells with X-ray irradiation.METHODS:The PC12 cells were irradiated with X-ray at doses of 2, 4 and 8 Gy.After 24 h, the levels of IL-1βand IL-6 were detected by ELISA .The protein levels of p-JAK1, p-JAK2, p-STAT1, p-STAT3 and p-STAT5 were measured by Western blot .RESULTS:Compared with control group , the levels of IL-1βand IL-6 increased .The protein levels of p-JAK1, p-JAK2, p-STAT1, p-STAT3 and p-STAT5 increased with the doses of X-ray exposed.CONCLUSION:JAK-STAT signaling pathway , IL-1βand IL-6 play a role in the injury of PC12 cells with X-ray irradiation.