Influence of mitogen-activated protein kinase inhibitor in the process of assemble flavone of rhizome drynaria promoting the osteogentic differentiation of myoblasts
10.3969/j.issn.2095-4344.2016.51.003
- VernacularTitle:MAPK抑制剂对骨碎补总黄酮促进转基因成肌细胞成骨分化的影响
- Author:
Li ZHANG
;
Boxing XU
;
Jie LI
;
Wei WANG
- From:Chinese Journal of Tissue Engineering Research
2016;20(51):7622-7627
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Previous studies have confirmed that drug-containing serum with low concentration of assemble flavone of rhizome drynaria (AFDR) promotes osteogentic differentiation of ciliary neurotrophic factor (CNTF)-modified myoblasts, but the underlying mechanism remains unclear.
OBJECTIVE:To observe the influence of mitogen-activated protein kinase (MAPK) inhibitor in the process of AFDR promoting the osteogentic differentiation of myoblasts.
METHODS:Transfected-CNTF myoblasts were preprocessed prior to osteogentic induction. Changes of alkaline phosphatase specific activities were detected in blank serum, AFDR drug serum and p38 pathway inhibitor SB203580 groups. mRNA and protein expression levels of core binding factor a1 and alkaline phosphatase were detected by real-time PCR and western blot, respectively.
RESULTS AND CONCLUSION:Real-time PCR showed that the mRNA expression levels of core binding factor a1 and alkaline phosphatase in the AFDR group were the highest;while p38 inhibitor SB203580 significantly downregulated the above levels. Western blot findings showed that a significant reduction in the protein expression levels of core binding factor a1 and alkaline phosphatase after p38 inhibitor SB203580 addition. These results suggest that p38 pathway inhibitor can downregulate osteogenesis-related gene and protein expressions, and thus AFDR promoting the osteogentic differentiation of CNTF-modified myoblasts probably through activating p38 MAPK signaling pathway.
