Effect of rapamycin on proliferation, apoptosis and cell cycles of high glucose-cultured rat glomerular mesangial cell
10.3969/j.issn.1000-484X.2017.01.009
- VernacularTitle:雷帕霉素对高糖诱导的大鼠肾系膜细胞增殖、凋亡和细胞周期的影响
- Author:
Jie CHEN
;
Xingqiang CHEN
;
Weiwei FU
- Keywords:
Rapamycin;
High glucose;
Glomerular mesangial cell;
Proliferation;
Apoptosis;
Cell cycle
- From:
Chinese Journal of Immunology
2017;33(1):47-51
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the effects of rapamycin on the proliferation,apoptosis and cell cycle of glomerular mesangial cells induced by high glucose,and to explore its significance in the prevention and treatment of diabetic nephropathy. Methods:The rat GMC HBZY-1 was divided into four groups:control group,high glucose group,the first group of high glucose plus rapamycin,the second group of high glucose plus rapamycin. CCK-8 assay was used to detect the proliferation of cells, flow cytometry was introduced to evaluate the apoptosis and cell cycle of HBZY-1,Real-time PCR was used to detect the mRNA of AngiotensinⅡ(ANGⅡ),transfor ming growth factor beta1 ( TGF-β1 ) and vascular endothelial growth factor ( VEGF ) . Results: The proliferation level of HBZY-1 induced by high glucose was significantly increased,and the level of apoptosis decreased,and the expression level of ANGⅡ,TGF-β1 and VEGF was increased. Rapamycin significantly inhibited,and there was a dose dependent,and down regulated the expression of ANGⅡ,TGF-β1,and VEGF. For the cell cycle,the S phase cells in the high glucose group were significantly higher than those in the normal group (P<0. 05),and the S phase cell proportion was decreased after rapamycin intervention (P<0. 05). Conclusion:Rapamycin can inhibit the proliferation of HBZY-1 in high glucose,promote its apoptosis and lead to G1/S arrest,and down regulate the expression of ANGⅡ,TGF-β1 and VEGF.
