Anti-aging Effect Study of Ethyl Acetate Extract from Folygonum multiflora on Caenorhabditis Elegans
10.6039/j.issn.1001-0408.2017.04.17
- VernacularTitle:何首乌乙酸乙酯提取物对秀丽线虫的抗衰老作用研究
- Author:
Jinfa TANG
;
Fan ZHANG
;
Yuhui LI
;
Suhui WU
;
Hanbing LI
;
Weixia LI
;
Xuelin LI
- Keywords:
Polygonum multiflora;
Ethyl acetate extract;
Anti-aging;
Caenorhabditis elegans;
Lifespan;
Oxidative stress;
Heat stress
- From:
China Pharmacy
2017;28(4):493-496
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the lifespan effect of ethyl acetate extract from Polygonum multiflora(EPM)on caenorhab-ditis elegans,and to investigate its anti-aging effect. METHODS:EPM low-concentration,medium-concentration and high- concen-tration groups(25,37.5,50 mg/L,calculated by crude drug)and blank group(0 mg/L)were established to investigate the effects of EPM on the lifespan of caenorhabditis elegans. EPM group(37.5 mg/L)and blank group(0 mg/L)were established to perform re-productive test,acute heat stress test and acute oxidative stress test of caenorhabditis elegans. The effects of EPM on reproductive capacity and pressure stress of caenorhabditis elegans were investigated. RESULTS:The lifespan test,average lifespan of cae-norhabditis elegans in EPM low-concentration,medium-concentration and high-concentration groups were prolonged significantly, compared to blank group(P<0.05 or P<0.01),especially in EPM medium-concentration group. In reproductive test,the number of offspring of caenorhabditis elegans in EPM group on the second and third day were increased significantly,compared to blank group (P<0.05). In acute heat stress test and acute oxidative stress test,average survival time of caenorhabditis elegans in EPM group was prolonged significantly(P<0.05). CONCLUSIONS:37.5 mg/L EPM can retard the aging process of caenorhabditis ele-gans and doesn’t damage the reproductive capacity.
