Simultaneous Determination of 7 Flavonoids in Fengliaochangweikang Capsules and Optimization of Ex-traction Process for Total Flavones
- VernacularTitle:枫蓼肠胃康胶囊中7种黄酮类成分的含量测定及总黄酮提取工艺的优化
- Author:
Rong XU
- Keywords:
HPLC;
Fengliaochangweikang capsules;
Flavonoids;
Content determination;
Orthogonal test;
Extraction process
- From:
China Pharmacist
2017;20(2):259-262
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish an HPLC method for the simultaneous determination of 7 flavonoids in Fengliaochangweikang capsules, and optimize the extraction process for total flavones. Methods:The extraction process was optimized by orthogonal test. The analysis was performed on a Waters RP18 column (250 mm × 4. 6 mm,5 μm). The mobile phase A was methanol-water (80 :20) and the mobile phase B was methanol-0. 2% formic acid (17 :83) with gradient elution. The flow rate was 1. 0 ml·min-1 and the column temperature was 35℃. The detection wavelength changed as follows:0-27 min,λ1 = 260 nm;27-60min,λ2 = 360 nm. Results:The 7 flavonoids obtained the baseline separation in 60 min, and the linear correlation coefficients were all above or equal to 0. 9900. The average recovery was 95. 95%-97. 84% and RSD was 0. 81%-1. 33%. The optimum extraction conditions were as follows:solid-liquid ratio was 1 :15,AB-8 macroporous adsorption resin was used, the ultrasonic extraction time was 30min, and the ethanol concentration was 60%. Conclusion:The established method has the advantages of simple operation, high precision and good repeatability,which shows certain guiding significance for the quality control and detection of Fengliaochangweikang capsules.
