Effects of Genistein on Inflammation and Apoptosis of Human Alveolar Epithelial Cell A549 Induced by Lipopolysaccharide
10.3969/j.issn.1005-5304.2017.03.014
- VernacularTitle:金雀异黄素对脂多糖诱导人肺泡上皮细胞A549炎症及凋亡的影响
- Author:
Ling WANG
;
Huiguo LIU
- Keywords:
Genistein;
acute lung injury;
A549;
inflammation;
apoptosis
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2017;24(3):57-60
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of Genistein (GEN) acting on human lung adenocarcinoma A549 cells induced by LPS;To discuss its action of mechanism for improving injuries of lung. Methods The activity of cell treated with different concentrations of Genistein was detected by CCK-8 method and/or LPS intervention for 12 h. The expressions of inflammatory cytokines IL-1β, IL-6, and TNF-α induced by LPS were detected by RT-PCR. TUNEL was used to detect apoptosis, and Western blot was used to detect the changes of signal pathway. Results GEN (1, 5, 10μmol/L) alone had no effects on cell activity;LPS (1μg/mL) reduced cell viability significantly, while co-treated the A549 cells with GEN and LPS could reverse this condition in a concentration-dependent manner;RT-PCR results showed that LPS could significantly increase the level of gene expression of inflammatory factors, while GEN could inhibited this phenomenon in both concentration-and time-dependent manner;the results of TUNEL staining showed that GEN (10μmol/L) combined with LPS for 12 h could significantly decrease the apoptosis rate;the results of Western blot showed that GEN possibly inhibited the inflammation and apoptosis through inhibiting the phosphorylation of IκBα, p65 signaling pathways. Conclusion GEN has anti-inflammation and anti-apoptosis effects on A549 cells induced by LPS, so as to play a protective rate.
