The expression of sex determining region Y-box 18 in prostate cancer and its impact on prostate cancer cell function
10.3760/cma.j.issn.1000-6702.2017.02.017
- VernacularTitle:性别决定区Y框蛋白18在前列腺癌中的表达及其对细胞功能的影响
- Author:
Luping YU
;
Chunlei LIU
;
Qing LI
;
Bo YANG
;
Lili CHEN
;
Shijun LIU
;
Tao XU
- Keywords:
Prostate cancer;
Sex determining region Y-box 18 (SOX18);
Small interfering RNA;
Cell proliferation;
Cell migration
- From:
Chinese Journal of Urology
2017;38(2):141-145
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the expression of sex determining region Y-box 18 (SOX18) in prostate cancer(PCa) and its role on prostate cancer cells.Methods The expression of SOX18 in 98 PCa tissues and 81 adjacent non-tumor tissues was detected by immunohistochemistry.The expression of SOX18 mRNA and protein in PCa cell lines were detected by q-PCR and Western blotting analysis,respectively.After knocking down SOX18 with si-RNA,the proliferation,migration and invasion of PCa cells were analyzed by CCK8,Transwell and Matrigel assays in vitro.Results The rate of high SOX18 expression in prostate cancer tissues was 73.5％ (72/98),which was higher than that in adjacent normal tissues (43.2％,35/81) (P ＜ 0.01).In addition,the rates of high SOX18 expression in clinical stage Ⅲ-Ⅳ [83.7 ％ (36/43) vs.65.5 ％ (36/55),x2 =4.131,P =0.042],pathological grading G3-4 [88.6％ (39/ 44) vs.61.7％ (33/54),x2 =9.424,P =0.002] and Gleason scores ≥ 8 [85.0％ (34/40) vs.65.0％ (38/58),x2 =4.610,P =0.032] groups were higher than those in clinical stage Ⅲ-Ⅳ,pathological grading G1-2 and Gleason scores ≤7 groups.However,there were no differences in the rate of high SOX18 expression in different age groups [77.3％ (38/54) vs.70.4％ (34/44),x2 =0.539,P =0.441].Knockdown of SOX18 notably suppressed the proliferation,migration and invasion of prostate cancer cells.Conclusions SOX18 is over-expressed in prostate cancer,and could promote the proliferation,migration and invasion ability of prostate cancer cells.
