Content Determination of Kaempferol in Kaempferia galanga by HPLC
10.6039/j.issn.1001-0408.2016.18.37
- VernacularTitle:HPLC法测定山柰药材中山柰素的含量
- Author:
Gang LI
;
Chuanhua FENG
;
Jing ZHANG
;
Lan XU
;
Xiaoxuan TAO
- Publication Type:Journal Article
- Keywords:
HPLC;
Kaempferia galanga;
Kaempferol
- From:
China Pharmacy
2016;27(18):2558-2559
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for the content determination of kaempferol in Kaempferia galanga. METH-ODS:HPLC was performed on the column of Diamonsil ODS2 C18 with mobile phase of methanol-0.4% Phosphoric acid solution at a flow rate of 1 ml/min,detection wavelength was 367 nm,column temperature was 30℃,and injection volume was 10 μl. RE-SULTS:The linear range of kaempferol was 0.001 58-0.158 mg/ml;RSDs of precision,stability and reproducibility tests were low-er than 3%;recovery was 95.52%-99.32%(RSD=1.47%,n=6). CONCLUSIONS:The method is simple,accurate and reproduc-ible,and can be used for the content determination of kaempferol in K. galanga.
