Optimization of the Purification Technology of Total Flavones in Ampelopsis grossedentala with Macropo-rous Resins by Central Composite Design-Response Surface Methodology
10.6039/j.issn.1001-0408.2016.07.24
- VernacularTitle:星点设计-响应面法优化大孔树脂纯化藤茶总黄酮的工艺
- Author:
Qinglong LIU
;
Huang LI
;
Zhucan LIN
;
Wei XU
;
Mei SHA
;
Ahong CHEN
;
Hourong ZHOU
- Publication Type:Journal Article
- Keywords:
D-101 macroporous resin;
Purification;
Ampelopsis grossedentata;
Total flavonids;
Central composite design-re-sponse surface methodology
- From:
China Pharmacy
2016;27(7):942-945
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To optimize the purification technology of total flavones from Ampelopsis grossedentala with macro-porous resins. METHODS:4 kinds of macroporous resins for the purification of total flavones from A. grossedentala were screened by using drug-loading amount,desorption rate,recovery and purification rate as indicators. Single factor test and central composite design-response surface methodology were used to optimized eluant mass fraction,adsorption time,flow rate of eluant,eluant pH and other factors of purification technology,and validation test was also conducted. RESULTS:D-101 macroporous resin was the best. The optimal condition was as follows as the concentration of sample solution 2 mg(by extract weight)/ml,the volume of sam-ple solution 1.1 BV,ethanol 86.0%,adsorption time 36.7 min,flow rate of eluant 3.81 BV/h,pH 7. In validation test,mass frac-tion of total flavones increased from 66.83% to 85.00% in validation test(RSD=0.15%,n=3),and were close to predicted val-ue(85.08%). CONCLUSIONS:Central composite design-response surface methodology is feasible and stable for the optimization of purification technology of total flavones from A. grossedentala with macroporous resins.
