Neuroprotective effects of sufentanil preconditioning on spinal cord injury in mouse models
10.3969/j.issn.2095-4344.2016.40.006
- VernacularTitle:舒芬太尼预处理对脊髓损伤模型小鼠的神经保护
- Author:
Yuqin YU
;
Nianchun HU
;
Jian DUAN
;
Dapeng LI
;
Chang LIU
- Publication Type:Journal Article
- Keywords:
Tissue Engineering;
Spinal Cord Injuries;
Models,Animal
- From:Chinese Journal of Tissue Engineering Research
2016;20(40):5966-5972
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Sufentanil exerts protective effects on tissues, but its roles in the repair of nervous system injury and the underlying mechanism are stil unknown.
OBJECTIVE:To explore the protective effect of sufentanil preconditioning in the repair of spinal cord injuries and the underlying mechanism.
METHODS:Mouse models of spinal cord injuries were prepared through clipping spinal cord fol owed by intraperitoneal injection of 3 and 6μg/kg sufentanil, respectively.
RESULTS AND CONCLUISON:(1) Western blotting, ELISA and TUNEL assays showed that 6μg/kg sufentanil significantly down-regulated the protein expression levels of TLR4, nuclear factor-κBp65, cleaved-caspase 3, tumor necrosis factor-αand interlenkin-1βin the spinal cord of mice (P<0.05);at the same time, the number of apoptotic neurons was significantly decreased (P<0.05). (2) Furthermore, high-dose sufentanil preconditioning significantly ameliorated the recovery of limb function at 14 days after injury (P<0.05). (3) These results administrate that the neuroprotection provided by 6μg/kg sufentanil preconditioning for spinal cord injuries in mice maybe related to the TLR4/nuclear factor-κB signaling pathway inactivation.
