Effect of advanced glycation end products on apoptosis of C57 mouse spiral ganglion cells and mRNA expression of advanced glycation end products receptor
10.16066/j.1672-7002.2016.10.010
- VernacularTitle:晚期糖基化终产物对C57小鼠耳蜗螺旋神经节细胞凋亡及其受体表达的影响
- Author:
Qilin GONG
;
Wenjing ZUO
;
Xiaobo WU
;
Chang LIN
- Publication Type:Journal Article
- Keywords:
Mice;
Animal Experinmentation;
Glycosylation End Products,Advanced;
Spiral Ganglion;
Cells,Cultured;
Apoptosis;
Presbycusis
- From:Chinese Archives of Otolaryngology-Head and Neck Surgery
2016;23(10):594-598
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To analyze the effect of advanced glycation end products(AGEs) on apoptosis of cultured mouse spiral ganglion cells(SGCs) and expression of receptor of AGEs(RAGE). To explore the pathway of AGEs in promoting apoptosis of SGCs. And to explore the possible mechanism of neural presbycusis. METHODS The effect of AGEs on apoptosis of SGCs was studied by Tunel technique and fluorescence microscope. The expression of RAGE mRNA was assayed by Real time RT-PCR. RESULTS AGEs induced apoptosis of cultured SGCs. The effects were dose-dependent and time-dependent. Meanwhile RAGE mRNA expression was enhanced in apoptosis cells. CONCLUSION AGEs induced apoptosis in SGCs,which may be mediated by RAGE. And this may be one of the mechanisms of neural presbycusis.
