Simultaneous Determination of Triamcinolone Acetonide Acetate and Tretinoin in Dimensional Ointment by HPLC
10.6039/j.issn.1001-0408.2016.03.32
- VernacularTitle:HPLC法同时测定维舒膏中醋酸曲安奈德和维A酸的含量
- Author:
Lili XIAO
;
Zhijun DING
;
Shigui XU
;
Yicheng GUO
;
Yamin WANG
;
Meilan LUO
- Publication Type:Journal Article
- Keywords:
HPLC;
Dimensional ointment;
Triamcinolone acetonide acetate;
Tretinoin;
Content determination
- From:
China Pharmacy
2016;27(3):381-383
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for the contents determination of triamcinolone acetonide acetate and tretinoin in Dimensional ointment. METHODS:HPLC was performed on the column of Diamonsil C18 with mobile phase of methanol-0.1%Phosphoric acid solution(87:13,V/V),detection wavelength was 254 nm(0-5 min)and 350 nm(5-20 min),at a flow rate of 1.0 ml/min,column temperature was 25℃,and volume injection was 10 μl. RESULTS:The linear range was 0.5-5 μg/ml for triamcin-olone acetonide acetate(r=0.999 9)and 4.96-49.6 μg/ml for tretinoin(r=0.999 8);RSDs of precision,stability and reproducibility tests were lower than 1%;recoveries were 98.37%-100.03%(RSD=0.58%,n=6)and 98.21%-100.38%(RSD=0.78%,n=6). CON-CLUSIONS:The method is simple,accurate and fast,and suitable for the contents determination of triamcinolone acetonide ace-tate and tretinoin in Dimensional ointment.
