Content Determination of Chlorogenic Acid in Yinhuang Qinghuo Capsule by HPLC
10.6039/j.issn.1001-0408.2015.36.38
- VernacularTitle:HPLC法测定银黄清火胶囊中绿原酸的含量
- Author:
Ligang XIE
;
Hejun CHEN
;
Shixue HE
;
Fang HE
- Publication Type:Journal Article
- Keywords:
Yinhuang qinghuo capsule;
Chlorogenic acid;
HPLC;
Content determination
- From:
China Pharmacy
2015;26(36):5147-5148
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method to determine the content of chlorogenic acid in Yinhuang qinghuo capsule. METHODS:HPLC was performed on the column of Acclaim120? C18 with mobile phase of acetonitrile-0.4% phosphoric acid(12:88,V/V)at flow rate of 1 ml/min,the detection wavelength was 327 nm,column temperature was 25 ℃,and volume injection was 10 μl. RESULTS:The linear range of chlorogenic acid was 56-224 μg;RSDs of precision,stability and reproduciblity tests were lower than 3.0%,recovery was 96.91%-104.76%(RSD=1.46%,n=9). CONCLUSIONS:The method is simple and reproducible, and can be used for the quality control of Yinhuang qinghuo capsule.
