Simultaneous Determination of Silybin A and Silybin B in Rat Plasma and Pharmacokinetic Study
10.3969/j.issn.1674-6384.2011.04.010
- Author:
Yang CHU
;
Wei LI
;
Zhiwen LI
;
Xinxin LI
;
Xiaohui MA
;
Shuiping ZHOU
;
Yonghong ZHU
- Publication Type:Journal Article
- Keywords:
bioavailability;
LC-MS/MS;
pharmacokinetics;
silybin A;
silybin B
- From:Chinese Herbal Medicines
2011;(4):304-309
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the bioavailability and pharmacokinetics of silybin A and silybin B in rats,respectively.Methods Following iv and ig administration of silybin to 20 Wistar rats,the plasma samples were collected at different time points up to 12 h.Sample pretreatment was involved in one-step protein precipitation with acetonitrile.Silybin A and silybin B were simultaneously determined by LC-MS/MS.Results After ig dosing silybin 28,56,and 112 mg/kg to rats,the t1/2β values were 5.48,5.08,and 5.73 h for silybin A,and 4.56,4.12,and 5.53 h for silybin B; The Cmax were 674.3,1349.4,and 2042.5 ng/mL for silybin A,and 671.0,1365.4,and 2066.2 ng/mL for silybin B; The Tmax were 0.20,0.23,and 0.20 h for silybin A,and 0.20,0.23,and 0.20 h for silybin B; The AUC were 454.4,845.9,and 1219.5 h·ng/mL for silybin A,and 432.0,817.1,and 1153.6 h·ng/mL for silybin B.The absolute bioavailabilities of silybin A and silybin B were 2.86％ and 1.93％,respectively.Conclusion Silybin A and silybin B have very low bioavailability after ig administration,and there is no significant difference in the pharmacokinetic parameters between silybin A and silybin B,which indicates that the two diastereoisomers have similar pharmacokinetic behavior in rats.
