Effects of berberine chloride on secondary brain injury in contralateral parietal lobe cortex of TBI model mice
10.3969/j.issn.1002-0152.2016.06.004
- VernacularTitle:小檗碱对颅脑创伤模型小鼠对侧顶叶皮层继发性损伤的影响
- Author:
Shuxuan HUANG
;
Feiqi ZHU
;
Zhong PEI
;
Jinhua ZHU
;
Zhi YANG
;
Xuhui DENG
;
Yuan LIU
- Publication Type:Journal Article
- Keywords:
Traumatic brain injury;
Berberine chloride;
Contralateral cortex;
Neuroinflammation;
Oxidative damage;
Neuron loss
- From:
Chinese Journal of Nervous and Mental Diseases
2016;42(6):338-341
- CountryChina
- Language:Chinese
-
Abstract:
Objective To examine neuroinflammation,oxidative damage and neuron loss in the contralateral parie-tal lobecortex of TBI model mice, and to investigate effects of berberine chloride on such secondary damage.Methods TBI model was established by a weight-drop hitting device and mice in berberine group were administered intragastrically with berberine chloride (50mg/kg.day) for 21 days.Immunofluorescence staining was used to assess activity of microglia and astrocyte.Immunohistochemistry was used to assess DNA oxidative damage, neuron loss and expression of COX-2 and iN-OS.Results Activation of microglia and astrocyte, expressions of COX-2 and iNOS and DNA oxidative damage were ob-viously increased by TBI,(19.82 ±1.88)and(16.96 ±1.69)、(13.79 ±4.32)and(8.67 ±0.96)、(27.86 ±5.38) and (16.00 ±7.59)、(31.92 ±6.57)and(24.79 ±2.78)respectively (P<0.01 or P<0.05).Activation of microglia and ex-pressions of COX-2 and iNOS were significantly suppressed by berberine ,(15.49 ±1.88)and(19.82 ±1.88)、(16.83 ± 7.89)and(27.86 ±5.38)、(26.25 ±2.41)and(31.92 ±6.57) respectively(P<0.01 or P<0.05).There was no differ-ence in neuron loss among three groups, (49.05 ±4.38),(48.56 ±3.56)and (47.75 ±4.14) respectively (P>0.05). Conclusions TBI can cause neuroinflammation and oxidative damage but not neuron loss in the contralateral parietal lobe cortex.Berberine chloride can significantly suppress neuroinflammtion in the contralateral parietal lobe cortex after TBI.
