Protective effect of loganin and morroniside on HUVEC injury induced by advanced glycation end products
10.3969/j.issn.1001-1978.2016.08.007
- VernacularTitle:山茱萸环烯醚萜苷类成分对 AGEs诱导 HUVEC 损伤的保护作用
- Author:
Hongsheng SHEN
;
Huiqin XU
;
Chunhong LU
;
Guoying DAI
;
Kang XU
;
Xing LYU
;
Yuping CHEN
;
Yunhao WU
- Publication Type:Journal Article
- Keywords:
Cornus officinalis Sieb.et Zucc.;
loga-nin;
morroniside;
diabetes mellitus;
AGEs;
HUVEC
- From:
Chinese Pharmacological Bulletin
2016;32(8):1063-1067
- CountryChina
- Language:Chinese
-
Abstract:
Aim To observe the protective mechanism of loganinand morroniside ( active components in Cornus officinalis) on HUVEC injury induced by advanced glycation end products ( AGEs ) .Methods HUVECs were cultured in vitro and divided into control group , model group ( AGEs group ) , loganin group , morroni-side group and aminoguanidine group ( set as positive control).After being incubated with loganin and mor-roniside( final concentrations were 100,10,1 μmol?L-1 ) for 1 h, HUVECs were stimulated by AGEs of 200 mg? L-1 for 24 h.Then, the cell viability was measured by using MTT method .The supernatant was extracted and the levels of NO ,ET-1,MCP-1,VCAM-1 were measured by the corresponding kits .Receptors of advanced glycation end products ( RAGE ) and NF-κB in HUVEC were detected by Western blot .Results Loganin and morroniside could inhibit HUVEC injury induced by AGEs .In model group ,the contents of ET-1,MCP-1,VCAM-1 increased(P<0.01),the content of NO decreased ( P <0.01 ) and the expression of RAGE and NF-κB increased(P<0.01); however,lo-ganin and morronside could reduce the ET-1,MCP-1, VCAM-1contents,increase the NO content and down-regulate the expression of RAGE and NF-κB to differ-ent extents .Conclusion Loganin and morroniside could ameliorate HUVEC injury , and its mechanism may be related to inhibit inflammation , the improve-ment of endothelial cell function , and the decrease of the expression of RAGE .
