Neuroprotective effect of active components of Xiaoxuming decoction on focal cerebral ischemia/reperfusion injury in rats during early recovery period
10.3969/j.issn.1001-1978.2016.07.011
- VernacularTitle:小续命汤有效成分组对局灶性脑缺血／再灌注大鼠恢复早期的神经保护作用研究
- Author:
Chang LU
;
Xiao DU
;
Xiaoli HE
;
Yuehua WANG
;
Guanhua DU
- Publication Type:Journal Article
- Keywords:
active components of XXMD;
cerebral is-chemia reperfusion;
recovery of neurological function;
neurological behavioral evaluation;
oxidative stress;
neuroprotective effect
- From:
Chinese Pharmacological Bulletin
2016;32(7):938-944
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the protective effect of ac-tive components of Xiaoxuming decoction ( XXMD ) on focal cerebral ischemia/reperfusion injury in rats dur-ing early recovery period .Methods The ischemia and reperfusion of middle cerebral artery model rats were established by nylon wire with 2 hours ischemic time on healthy male SD rats .The models of MCAO were eval-uated by Zea-Longa′s standard score .The model rats were randomly divided into sham operation group , the ischemia/reperfusion model group , the active compo-nents group of Xiaoxuming decoction and the positive group (extract of ginkgo biloba leaves EGb 761).Rats were orally administrated with different drugs 24 h after operation for up to 14 days, once a day.The effect of active components of Xiaoxuming decoction on behavior changes of MCAO rats in different recovery period was evaluated by a series of behavioral assessent methods such as modified neurological severity scores and cor-ner test.The infarct volume was observed by TTC stai-ning.Moreover, the contents of MDA and the activities of GSH-Px, SOD and NOS in the penumbra and core tissues of rat brain were detected by spectrophotometric method.Results Compared with the I/R model group, the active components group of XXMD could significantly alleviate the neurological deficit scores with prolonged administration . The motion function tended to be normal , stayed longer on the balance beam, sensory function gradually restored sensitivity , reduced the radio of turning to the right .Among them , compared with model group , the active components of XXMD could effectively improve the neurological dys-function after five days of administration ( P<0.05, P<0.01).Meanwhile, the active components of Xiaox-uming decoction could significantly reduce the infarct volume percentage in the cerebral tissue on post opera-tion day 5 and 14(P<0.01).In addition, the active components of XXMD could reduce the content of MDA and the activity of NOS , increase the activity of SOD and GSH-Px.Conclusion The active components of XXMD can generate neuroprotective effect in early stage of recovery and may play a major role in regula-ting the level of oxidative stress in rat brain .
