Determination of Chlorogenic Acid and Rutin in Tibetan Medicine Nettle by HPLC
- VernacularTitle:藏药材荨麻中绿原酸和芦丁的含量测定
- Author:
Duanwei ZHAO
;
Shusheng YANG
;
Jianbang ZHAO
;
Pingshun SONG
- Publication Type:Journal Article
- Keywords:
Nettle;
Chlorogenic acid;
Rutin;
Content determination
- From:
China Pharmacist
2016;19(7):1409-1410
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a method to determine the content of chlorogenic acid and rutin in nettle to assess the quality of nettle.Methods:An HPLC method was used with the following chromatographic conditions:CAPCELL PAK C18 column (250 mm × 4.6 mm, 5 μm) was used with the mixture of 0.4%phosphoric acid and methanol as the mobile phase with gradient elution , the de-tection wavelength was set at 340 nm, the flow rate was 1.0 ml· min-1 and the sample size was 10μl.Results:Chlorogenic acid with-in the range of 2.360-23.600μg · ml-1 showed a good linear relationship (r=0.999 9), and rutin within the range of 6.208-62.080μg · ml-1 showed a good linear relationship (r=0.999 9).The recovery of rutin and chlorogenic acid was 99.20% and 100.40%with RSD of 1.2%and 1.1%(n=6), respectively.Conclusion:The method is fast, effective, simple, accurate and reproducible , which can be used to quantitatively analyze chlorogenic acid and rutin in nettle .
