Effect of gambogic acid on cell apoptosis and expressions of Bax, Bcl-2 and Caspase-3 in colorectal cancer cells with
10.3969/j.issn.1006-5725.2016.11.006
- VernacularTitle:藤黄酸对人结直肠癌细胞凋亡及Bax、Bcl-2和Caspase-3表达的影响
- Author:
Jianchang WEI
;
Tong ZHANG
;
Ping YANG
;
Shanqi ZENG
;
Chengxing WANG
;
Huacui CHEN
;
Jie CAO
- Publication Type:Journal Article
- Keywords:
colorectal neoplasms;
Gmbogic acid;
Apoptosis;
Bax/Bcl-2;
Caspase-3
- From:
The Journal of Practical Medicine
2016;32(11):1745-1748
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investiagate cell apoptosis and expressions of Bax,Bcl-2 andCaspase-3 in gambogic acid-treated colorectal cancer cells. Methods SW480/LOVO colorectal cancer cells were treated by gambogic acid. Cell Counting Kit-8 assay (CCK-8) was used to test cell proliferation. Microscopy was used to check the morphological changes. Immunofluorescence staining technique was used to detect cell apoptosis. Expressions of Bax,Bcl-2 and Caspase-3 protein were detected by Western blot assay. Results Gambogic acid inhibited the proliferation of SW480/LOVO in a dose and time-dependent manner. Gambogic acid could induce cell apoptosis. Gambogic acid increased expressions of Caspase-3 and Bax, increased the ratio of Bax/Bcl-2, and decreased Bcl-2 protein expression. Conclusion Gambogic acid can inhibit proliferation and induce apoptosis of SW480LOVO cells, with the mechanism of up-regulation of Bax/Bcl-2 and activation of Caspase-3.
