Radiosensitizing effect of genistein on laryngeal carcinoma Hep-2 cells

VernacularTitle:染料木黄酮增强喉癌Hep-2细胞放射敏感性的研究
Author: Huimin WANG ; Yu ZHOU ; Shuxiang ZHANG
Publication Type:Journal Article
Keywords: Radiation Tolerance; Tumor Cells,Cultured; genistein
From: Chinese Archives of Otolaryngology-Head and Neck Surgery 2016;23(5):259-262
CountryChina
Language:Chinese
Abstract: OBJECTIVETo investigate whether the genistein can increase the radiosensitizing effect on laryngeal squamous carcinoma Hep-2 cells.METHODS Hep-2 cells were treated with genistein, radiation, and genistein plus radiation respectively. DMSO was used as the control group. EdU assay was performed to assess the short-term effect of genistein and (or) radiation on the proliferation of Hep-2 cells. Clonegenic assay was used to detect the survival rate of Hep-2 cells after treatment with radiation doses of 0, 2, 4, 6, 8 Gy and radiation combined with genistein. The data was fitted into the classic single-hit multi-target mathematical model to analyze the long-term effect on cell proliferation death of Hep-2 cells.RESULTSIt was observed that radiation combined with genistein could significantly inhibit the proliferation of Hep-2 cells. And the SER of 10μmol/L genistein was 1.412.CONCLUTIONGenistein can inhibit the proliferation of Hep-2 cells by DNA synthesis inhibition, and can be an adjunct agent of radiotherapy.