The protective effects of glutathione on renal oxidative damage induced by microcystin-LR on mice
10.3969/j.issn.1671-8348.2016.03.010
- VernacularTitle:谷胱甘肽对微囊藻毒素-LR所致小鼠肾脏氧化损伤的保护作用研究
- Author:
Zhixia HAN
;
Meiyan HE
;
Yan CUI
;
Wei XIONG
;
Qingbi ZHANG
- Publication Type:Journal Article
- Keywords:
kidney;
glutathione;
microcystin-LR;
oxidative damage;
protective effect
- From:
Chongqing Medicine
2016;(3):320-322
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the protection of glutathione (GSH) on renal oxidative damage to mice which caused by mi‐crocystin‐LR(MC‐LR) .Methods Forty healthy KM mice were divided into five groups by randomly sampling ,which were saline control group ,GSH control group ,MC‐LR group ,low dose GSH +MC‐LR group and high dose GSH +MC‐LR group ,and the ex‐periment was lasting 15 days by intraperitoneal injection .Then we took out the kidney for pathological observation and detected the activity of CAT ,SOD ,GSH‐Px and the content of GSH ,MDA .Results Compared with control group ,the MC‐LR increased the content of MDA[(2 .31 ± 0 .22)nmol/mg prot ,P=0 .000] and decreased the content of GSH[(0 .68 ± 0 .02)mg/g prot] .The activi‐ty of CAT[(320 .54 ± 38 .99)nmol/mg prot] ,SOD[(180 .93 ± 15 .30)U/mg prot] ,GSH‐Px[(295 .11 ± 42 .40)U/mg prot](P<0 .05) .However ,after GSH was given ,compared with MC‐LR group ,MDA content[(1 .94 ± 0 .12)nmol/mg prot]of high dose GSH+MC‐LR group significantly decreased (P<0 .05) ,GSH content[(1 .01 ± 0 .08)mg/g prot ,(1 .08 ± 0 .16)mg/g prot]and CAT activity[(383 .46 ± 21 .98)nmol/mg prot ,(428 .50 ± 28 .61)nmol/mg prot] of both GSH groups significantly increased (P<0 .05) ,the activity of SOD[(222 .01 ± 11 .51)U/mg prot] and GSH‐Px[(358 .37 ± 20 .29)U/mg prot] of high dose GSH +MC‐LR group significantly increased (P<0 .05) .Conclusion MC‐LR may cause renal oxidative damage through promoting the lipid perox‐idation on renal cells .The GSH may reach a certain protective effect on kidney by reducing the lipid peroxidation ,improving the an‐tioxidant activity ,and removing oxygen free radicals .
