Application of basic fibroblast growth factor gene-transfected bone marrow mesenchymal stem cells in denervated muscle atrophy
10.3969/j.issn.2095-4344.2016.01.016
- VernacularTitle:碱性成纤维细胞生长因子基因转染骨髓间充质干细胞在失神经肌肉萎缩中的应用
- Author:
Ning YU
;
Yansheng WANG
;
Changping QI
- Publication Type:Journal Article
- Keywords:
Bone Marrow;
Mesenchymal Stem Cel Transplantation;
Fibroblast Growth Factor 2;
Muscular Disorders,Atrophic;
Tissue Engineering
- From:
Chinese Journal of Tissue Engineering Research
2016;20(1):89-94
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:How to avoid denervated muscular atrophy is a key factor to improve the therapeutic efficacy on peripheral nerve injuries. OBJECTIVE:To study the effect of basic fibroblast growth factor (bFGF) gene-transfected bone marrow mesenchymal stem cels against denervated muscle atrophy. METHODS: bFGF genes were transfected into rat bone marrow mesenchymal stem cels using viral transfection method, and then MTT, immunohistochemical staining, hematoxylin-eosin staining, RT-PCR, western blot, and ELISA methods were used to detect the transfection efficiency and product expression. Thirty-two Sprague-Dawley rats were selected to make animal models of sciatic nerve injury, and subjected to multi-point intramuscular injection of bFGF-transfected bone marrow mesenchymal stem cels (experimental group) or cel culture fluid (control group). At 2, 4, 6, 8 weeks after transfection, the gastrocnemius muscle tissues were harvested to detect action potential, residual wet weight, and cross-sectional area of muscle fibers. RESULTS AND CONCLUSION:The bFGF gene was successfuly transfected into bone marrow mesenchymal stem cels using the viral transfection method. The residual wet weight, cross-sectional area and residual action potential of the gastrocnemius muscle were significantly better in the experimental group than the control group (P < 0.05). These findings indicate that bFGF gene-transfected bone marrow mesenchymal stem cels transplanted into the denervated muscle can retard the development of muscle atrophy. Cite this article:Yu N, Wang YS, Qi CP.Application of basic fibroblast growth factor gene-transfected bone marrow mesenchymal stem cels in denervated muscle atrophy. Zhongguo Zuzhi Gongcheng Yanjiu. 2016;20(1):89-94.
