Genistein inhibits proliferation of human oral cancer TCA8113 cells through suppression of VEGF expression
10.3969/j.issn.1000-4718.2016.03.013
- VernacularTitle:染料木黄酮通过抑制 VEGF 表达抑制人口腔癌 TCA8113细胞增殖
- Author:
Hongliang DAI
;
Shuqing GE
;
Minghui CHU
;
Chunguang LIANG
;
Yue WANG
;
Guizhi JIA
- Publication Type:Journal Article
- Keywords:
Genistein;
TCA8113 cell;
Cell proliferation;
Vascular endothelial growth factor;
Extracellular signal-regulated kinase
- From:
Chinese Journal of Pathophysiology
2016;32(3):464-469
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effect of genistein on the proliferation of human oral cancer TCA 8113 cells and to explore the underlying mechanisms .METHODS:The cell proliferation was examined by MTT assay , cell counting and colony formation assay .Western blotting was employed to examine the protein levels of vascular endothelial growth fac -tor (VEGF), extracellular signal-regulated kinase (ERK) and p-ERK.RESULTS: Genistein significantly inhibited the proliferation of TCA8113 cells in a concentration-dependent fashion .Moreover , genistein dose-dependently decreased the protein levels of VEGF, ERK and p-ERK.The expression of VEGF was also blunted by U 0126, a specific inhibitor of ERK.U0126 and axitinib, a VEGF receptor antagonist , both significantly inhibited the proliferation of TCA 8113 cells. CONCLUSION:Genistein inhibits the proliferation of TCA8113 cells, which may be related to its inhibitory effect on ERK expression and activation , thus subsequently decreasing the expression of VEGF .
