The impact of celastrol on cognitive function and expressions of Aβ40 and Aβ42 in hippocampus in APPswe/PS1dE9 double transgenic mouse after partial hepatolobectomy
10.3760/cma.j.issn.1008-1372.2015.11.022
- VernacularTitle:雷公藤红素对APPswe/PS1dE9双转基因AD模型小鼠肝叶部分切除术后空间学习记忆能力及海马Aβ表达的影响
- Author:
Liyan SHI
;
Yanjie WAN
;
Fangfang XU
;
Yuxi CAI
;
Jing XU
- Publication Type:Journal Article
- Keywords:
TRIPTERYGIUM WILFORDII△/CH/PD;
Alzheimer disease/SU/ZD/ME;
Mice,transgenic;
Hepatectomy;
Learning/DE;
Memory/DE;
Hippocampus/ME;
Amyloid beta-protein/ME
- From:
Journal of Chinese Physician
2015;17(11):1676-1679
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of celastrol on space learning capability and expressions of beta-amyloid (Aβ) 40 and Aβ42 in hippocampus in APPswe/PS1dE9 double transgenic mouse after partial hepatolobectomy.Methods The 3-month-old APPswe/PS1dE9 double transgenic mice (n =96) were randomly divided into three groups according to the random number table method.Surgery group (group S, partial hepatolobectomy;n =32), celastrol group (group C, injections of dimethyl sulphoxide/DMSO and celastrol for 3 days before undergoing partial hepatectomy, on the surgery day, and for a further 4 days after surgery;n =32), and DMSO group (group D, injections of DMSO for 3 days before undergoing partial hepatectomy, on the surgery day, and for a further 4 days after surgery;n =32).Eight mice were selected randomly in each group and were Morris-water maze trained for continuous 5 days.Theirs learning and memory abilities were evaluated at 1,3, 7 and 14 d after surgery, respectively.Hippocampus was collected and the changes of β40 and Aβ42 were measured by enzyme-linked immunosorbent assay (ELISA) at the time set in advance in each group.Results The average escape latency of group C was significantly shorter than groups S and D at 3, 7 and 14 d after partial hepatectomy (P ＜ 0.05).Times of passing through the platform groups S and D were significantly less than group C (P ＜ 0.05).The expressions of Aβ40/Aβ42 in group C were lower than group S and group D at 1, 3, 7 and 14 d after partial hepatectomy (P ＜ 0.05).Conclusions Through decreasing the expressions of Aβ40 and Aβ42 in hippocampus,celastrol improves the space learning capability in APPswe/PS1dE9, the double transgenic mouse after partial hepatolobectomy.
