Characterization of one strain of acid-fasting Propionibacterium isolated from the breast abscess samples
10.3969/j.issn.1673-4130.2016.09.026
- VernacularTitle:1株抗酸阳性丙酸杆菌的分离和鉴定
- Author:
Qingming JIANG
;
Pinghua QU
;
Cha CHEN
;
Morui CHEN
- Publication Type:Journal Article
- Keywords:
Propionibacterium;
acid-fast;
bacteria identification;
sequence analysis
- From:International Journal of Laboratory Medicine
2016;37(9):1219-1220
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify one acid‐fasting bacteria isolated from wound secretion of breast abscess .Methods The acid‐fasting strain was identified by the morphological characteristics ,API 20A strips ,classical biochemical reaction ,and 16S rRNA gene sequencing .Results Cells of the strain was anaerobic ,non‐spore‐forming ,pleomorphic ,straight or curved rods ,which Gram and acid‐fast stain both were positive .After incubation for 5 days on sheep blood agar ,colonies were than 2 mm in diameter ,circular , smooth ,entire ,bump ,rice cream‐like withβ‐hemolysis .The 16S rRNA gene sequences were 100% identity to Propionibacterium av‐idum .The API 20A profile was 44365062 with positive Voges‐Proskauer test ,which was also consistent to Propionibacterium avi‐dum .Conclusion The pathogens of breast abscess is Proionibacterium avidum ,which is the first acid‐fasting Propionibacterium re‐ported in China .
