Effects of wedelolactone inhibiting expression of P-glycoprotein in K562/A02 cells
10.3760/cma.j.issn.1673-4246.2015.12.011
- VernacularTitle:蟛蜞菊内酯抑制K562/A02细胞P-糖蛋白的表达
- Author:
Ziling LUO
;
Jie CHEN
;
Jing XU
;
Biqiong GUAN
;
Binghong HE
- Publication Type:Journal Article
- Keywords:
Leukemia;
Wedelolactone;
Drug resistance,multiple;
P-glycoprotein
- From:
International Journal of Traditional Chinese Medicine
2015;37(12):1101-1104
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of wedelolactone on the expression of P-glycoprotein (P-gp) and to explore the multi-drug resistance reversing mechanism of wedelolactone in K562/A02 cells in vitro.Methods The half maximal inhibitory concentration of ADM in K562/A02 was determined by MTT method.The protein expression level of P-gp was determined by Western blot after wedelolactone pretreatment.Results Wedelolactone remarkably enhanced chemo-sensitivity to ADM of K562/A02 cells.After 0.2, 2, 20 μmol/L different concentration of wedelolactone treatment in 24 h, the relative reversal efficiency of K562/A02 to ADM was 23.5％, 47.1％ and 67.7％, respectively.according to the results of Western blot, wedelolactone was shown to efficiently inhibit the expression of P-gp (P＜0.05).The relative efficiency of K562/A02 to ADM was 25.4％,46％ and 55.6％, respectively.Conclusion Wedelolactone could modulate P-gp expression, and P-gp expression down regulation may be one of the MDR reversal mechanisms in K562/A02 cells by wedelolactone.
