Sulforaphane suppressed the proliferation of glioma stem cells via miR-124
10.11958/20150197
- VernacularTitle:莱菔硫烷经由miR-124抑制SWO-38胶质瘤干细胞的增殖
- Author:
Youke XIE
;
Xuemei LI
;
Dingping HUANG
- Publication Type:Journal Article
- Keywords:
glioma;
neoplastic stem cells;
cell proliferation;
microRNAs;
sulforaphane;
miR-124
- From:
Tianjin Medical Journal
2016;44(3):306-310
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of sulfuraphane (SFN) on proliferation of glioma stem cell line SWO-38, and its mechanism threreof. Methods Cell proliferation of SWO-38 treated with SFN was measured by cell prolifera?tion assay. Clone formation experiment, tumor sphere formation experiment and Western blotting method were applied to de?tect the ability of cell clone formation and tumor sphere formation, and the expression of stemness relative genes, such asβ-catenin, Oct4, Sox-2 and c-Myc. The effects of SFN and/or miR-124 inhibitor (miR-124i) on the expression of stemness rel?ative genes were compared. Changes of miRNAs (miRNA-9, 21, 221, 124, 128 and 181) induced by SFN were measured by real time quantity PCR. Results SFN suppressed the proliferation of SWO-38 cells in a dose-dependent manner, in which IC50 was (26.41±2.13)μmol/L. SFN also decreased the ability of forming cell clone and tumor sphere, as well as the expres?sion of stemness relative genes (β-catenin, Oct4, Sox-2 and c-Myc) in a dose-dependent manner. At the same time, SFN led to the change in many miRNAs, during which SFN increased the transcription of miR-124 (-5.9-fold) and miR-128 (-2.6-fold), and decreased the transcription of miR-9, miR-21 and miR-221. Compared to the blank control, the expression levels ofβ-catenin, Oct4, and Sox-2 were significantly increased in miR-124i group. On the other hand, the expressions of above genes were also higher in combined group, which was treated with miR-124i and SFN than those in SFN group, but lower than those of miR-124i group. Conclusion SFN can efficiently inhibit the proliferation of giloma stem cells in SWO-38 cell line through miR-124/(β-catenin/Sox-2/Oct4) signaling pathway.
