Neuroprotective effects of blonanserin on H2O2-induced injury in PC12 cells
10.11958/20150159
- VernacularTitle:布南色林对H2O2引起的PC12细胞损伤的神经保护作用研究
- Author:
Haichao HUANG
;
Wei WU
;
Xiaohong ZHANG
;
Yang NIE
;
Jingliang LIU
;
Jie ZHOU
- Publication Type:Journal Article
- Keywords:
trauma,nervous system;
apoptosis;
PC12 cell;
neuroprotective;
Blonanserin;
SOD;
MDA
- From:
Tianjin Medical Journal
2016;44(3):311-313
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore neuroprotective effects of blonanserin on H2O2-induced injury in PC12 cells. Meth?ods PC12 cells were divided into four groups:control group (C group), H2O2-treated group (H group), blonanserin pretreat?ed group (B group) and positive control group (vitamin E- pretreated, E group). The effects of different concentrations of blonanserin (0, 5, 10, 20, 40, 80 and 160 μmol·L-1) on cell proliferation in PC 12 cells were observed. MTT assay was used to detect the cell activity of different groups. The apoptotic rates of different groups were measured by TUNEL assay. The mor?phological changes were observed using inverted microscope and Hoechst 33258 staining. The superoxide dismutase (SOD) vi?ability and malondialdehyde (MDA) levels were detecded by biochemical methods in four groups. Results The appropriate concentration of blonanserin (0-20 μmol·L-1) can promote the growth of PC12 cells. Comparing with the C group, the apoptot?ic rate and MDA level were increased in group H, while the cell viability and the SOD viability were decreased obviously ( P<0.05). Compared with H group, the cell viability, SOD viability were significantly increased, while the MDA level and apoptotic rate were decreased (P<0.05). Conclusion Blonanserin shows neuroprotective effect on H2O2-induced injury in PC12 cells.
