Protective effect of loganin on podocyte injury induced by advanced glycation end products
10.3969/j.issn.1001-1978.2016.03.008
- VernacularTitle:马钱苷对糖基化终末产物诱导足细胞损伤的保护作用
- Author:
Yunhao WU
;
Yuping CHEN
;
Xing LYU
;
Zhiruo ZHOU
;
Hongsheng SHEN
;
Guoying DAI
;
Huiqin XU
- Publication Type:Journal Article
- Keywords:
Cornus officinalis Sieb. et Zucc.;
loga-nin;
advanced glycation end products;
podocyte;
dia-betic nephropathy;
apoptosis
- From:
Chinese Pharmacological Bulletin
2016;32(3):332-336
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the protective effect of lo-ganin ( an active component in Cornus officinalis ) on podocyte injury induced by advanced glycation end products ( AGEs) and its possible mechanism. Meth-ods Mouse podocytes were cultured in vitro and di-vided into Normal group, model group ( AGEs group) , loganin group and aminoguanidine group ( set as posi-tive control) . After being incubated with loganin( final concentrations are 0. 1, 1, 10 μmol · L-1 ) for 1 h, podocytes were stimulated by AGEs of 100 mg · L-1 for 24 h. Then, the cell viability was measured by u-sing MTT method. Podocytes apoptosis was evaluated by Hoechst33342/PI staining and flow cytometry. Re-ceptors of advanced glycation end products ( RAGE ) ,desmin and apoptosis-related protein like Bax, Bcl-2, cleaved caspase-3 in podocytes were detected by Western blot. Results Loganin ameliorated podocyte injury induced by AGEs, down-regulated the expression of desmin and RAGE. Loganin also reduced the apoptotic rate of podocytes and decreased the ratio of Bax/ Bcl-2 and the expression of pro-apoptotic protein cleaved caspase-3 in podocytes. Conclusion Loganin could ameliorate podocyte injury, and its mechanism may be related to the decrease of the expression of RAGE and inhibition of the apoptotic pathway.
