Anti-inflammatory effect of Chikusetsu oleanane saponin on RAW264. 7 cell through regulating SIRT1 activity

VernacularTitle:竹节参齐墩果烷皂苷对RAW264．7巨噬细胞SIRT1活性影响及抗炎作用研究
Author: Qin YUAN ; Ding YUAN ; Zhiyong ZHOU ; Chaoqi LIU ; Ting WANG ; Tingting XIANG ; Changcheng ZHANG
Publication Type:Journal Article
Keywords: COS; RAW264. 7 cells; LPS; NO; TNF-α; IL-1β; NF-κB; SIRT1
From: Chinese Pharmacological Bulletin 2016;32(3):349-354
CountryChina
Language:Chinese
Abstract: Aim To investigate SIRT1 activity and an-ti-inflammatory effects of Chikusetsu oleanane saponin ( COS) on the RAW264. 7 macrophage cells induced by lipopolysaccharide ( LPS ) . Methods The nitric oxide ( NO) release was detected with Griess method. Western blot was used to detect the expression of tumor necrosis factor-α( TNF-α) , interleukin 1β( IL-1β) . Nuclear factor-κB ( NF-κB) and silent information ad-justment factor 1 ( SIRT1 ) were tested by immunofluo-rescence. Results 1 mg · L-1 LPS co-cultured with COS at 25~300 mg·L-1 had no significant effect on the growth of RAW264. 7 cells. Compared with the LPS group, COS effectively inhibited the NO release and suppressed the expression of TNF-α and IL-1β, and also inhibited the translocation of NF-κB and up-regulation of SIRT1 . Conclusion COS has protective effects on RAW264. 7 cells stimulated by LPS, which may be related to up-regulating the expression of SIRT1 and promoting the deacetylation of NF-κB, thereby in-hibiting the translocation of NF-κB and reducing the expression of TNF-α and IL-1β.