Protective effect of rhein on aristolochic acid-induced renal injury in zebrafish
10.3969/j.issn.1001-1978.2016.03.013
- VernacularTitle:大黄酸对马兜铃酸A引起的斑马鱼肾脏损伤的保护作用
- Author:
Xue WANG
;
Kechun LIU
;
Ximin WANG
;
Liwen HAN
;
Shanshan ZHANG
;
Qiuxia HE
;
Xiqiang CHEN
;
Jian HAN
;
Rongchun WANG
- Publication Type:Journal Article
- Keywords:
aristolochic acid;
zebrafish;
larvae;
kid-ney;
rhein;
creatinine
- From:
Chinese Pharmacological Bulletin
2016;32(3):361-365
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the effect of rhein on renal damage induced by aristolochic acid. Methods Ze-brafish model of aristolochic acid nephropathy, genera-ted by treating zebrafish larvae with aristolochic acid for 24 h, was treated with rhein simultaneously . Mor-pholigical changes were observed and the creatinine level in larvae tissue was measured. And mRNA ex-pression levels of inflammatory factor cox2 a and fibrosis factor TGF-β1 in larvae tissue were detected using qPCR. Results Some larvae show periocular edema and circulation system defection e. g. weak heart beat, narrow cardiac vesicle, decreased blood flow and even blockage , with a dose-response relationship after expo-sure to aristolochic acid for 24 h. The creatinine level in larvae tissue of the treated group was significantly higher than that of the control larvae. And the expres-sion levels of cox2 a and TGF-β1 in larvae tissue of the treated group were also significantly increased. Per-centage of abnormal larvae and creatinine level in lar-vae tissue were decreased when treated with rhein sim-ultaneously. And the expression levels of cox2a was down-regulated by rhein compared with the aristolochic acid treated group. But rhein had no effect on TGF-β1 expression. Conclusion To some extent rhein can protect renal from damage induced by aristolochic acid.
