Expression of silence information regulator 1 (Sirt1) in B cells in peripheral venous blood of subacute cutaneous lupus erythematosus

VernacularTitle:亚急性皮肤型红斑狼疮患者外周血B细胞组蛋白修饰酶Sirt1的表达水平研究
Author: Nan HU ; Qing LI ; Zhiping XIAO ; Qin QIN ; Cuie KUANG
Publication Type:Journal Article
Keywords: Lupus erythematosus,systemic/ME/GE; Sirtuins/ME/GE; B-lymphocytes
From: Journal of Chinese Physician 2015;17(12):1836-1838
CountryChina
Language:Chinese
Abstract: Objective To investigate alterations in epigenetic regulation in B cells in subacute cutaneous lupus erythematosus (SCLE).Methods Peripheral blood mononuclear cells (PBMC) were isolated from the peripheral venous blood of SCLE patients and healthy donors with density gradient centrifugation.B cells were isolated with CD19 micro beads and protocols provided by the manufacturer.Total RNAs were isolated with RNA kits.The expressions of silence information regulator 1 (Sirt1) mRNA were investigated with quantitative real time polymerase chain reaction (PCR).Results The levels of Sirt1 mRNA were significantly increased in SCLE B cells relative to healthy controls (P ＜ 0.01).Conclusions Histone modifications appear abnormal in B cells in SCLE.