Study on Preparation of Celastrol Ethosome and Its Skin Penetration Properties in Vitro
10.13359/j.cnki.gzxbtcm.2015.05.032
- VernacularTitle:雷公藤红素醇质体的制备及体外透皮性能研究
- Author:
Jun WU
;
Ming WU
;
Di LIU
;
Zhuo MA
- Publication Type:Journal Article
- Keywords:
Celastrol/production&preparation;
Ethosomes/ultrastructure;
Encapsulation efficiency;
Percutaneous penetration in vitro;
Disease models,animal;
Mice
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2015;(5):929-933
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare celastrol ethosomes and to observe the permeability characteristics of the ethosomes which act as the transdermal delivery carriers of celastrol in vitro. Methods Celastrol ethosomes were prepared by ethanol injection method, and then the encapsulation efficiency, particle size, polydispersity index ( PDI) and zeta potential of the ethosomes were analyzed. TP2A intelligent percutaneous penetration instrument was used to compare the skin penetration properties of celastrol ethosomes, celastrol solution and the mixture of blank ethosomes with celastrol solution. Results The prepared celastrol ethosomes were spherical, and the average particle size was (401.3 ± 5.5) nm, PDI was 0.21± 0.02, steady zeta potential was (-2.75 ± 0.1) mV, and average encapsulation efficiency was ( 80.6 ± 0.7) %. The amount of accumulative penetration of celastrol ethosomes at 48 h was 76.86 μg·cm -2 and the permeation rate was 1.640 9 μg·cm -2·h -1, which were significantly higher than the celastrol solution and the mixture of blank ethosomes with celastrol solution. Conclusion The prepared ethosomes have high encapsulation efficiency, uniform particle size and stable quality, and are beneficial to the transdermal absorption of celastrol.
