Simultaneous Determination of Triamcinolone Acetonide Acetate and Chloramphenicol in Chloramphenicol and Triamcinolone Acetonide Acetate Cream by HPLC-DAD
- VernacularTitle:HPLC法同时测定氯霉素醋酸曲安奈德乳膏中两组分含量
- Author:
Ping YANG
;
Weixin ZENG
;
Lulu SUN
- Publication Type:Journal Article
- Keywords:
Triamcinolone acetonide acetonide;
Chloramphenicol;
Gradient elution;
Content determination
- From:
China Pharmacist
2016;19(3):591-593
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish an HPLC method for the simultaneous determination of triamcinolone acetonide acetonide ( TAA) and chloramphenicol in the cream. Methods:The chromatographic system consisted of a ZORBAX SB-C18 column (250 mm × 4. 6 mm, 5μm). The mobile phase consisted of methanol and phosphate buffer with gradient elution at a flow rate of 1. 0 ml·min-1, the detection wavelength was 240nm, the column temperature was ambient, and the sample size was 20 μl. Results:The calibration curve was linear for TAA and chloramphenicol within the concentration range of 6. 12-48. 96 μg·ml-1 and 62. 1-745. 2 μg·ml-1 with the recovery of 99. 7% (RSD=1. 3%, n=9) and 99. 4%(RSD=1. 0%, n=9), respectively. Conclusion: The method is sensitive, accurate and specific, and can be used to control the quality of chloramphenicol and triamcinolone acetonide acetate cream.
