The impact of miR-320-3p on adipocyte differentiation
10.11958/j.issn.0253-9896.2015.12.003
- VernacularTitle:miR-320-3p调控脂肪细胞分化的研究
- Author:
Changlan WANG
;
Zhihong GAO
;
Ailing CHANG
;
Baoli WANG
- Publication Type:Journal Article
- Keywords:
adipocytes;
mesenchymal stem cells;
cell differentiation;
adipogenesis;
microRNAs;
miR-320-3p
- From:
Tianjin Medical Journal
2015;(12):1353-1355
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the role of miR-320-3p in adipocyte differentiation. Methods Marrow mesenchymal stem cells were isolated from mice and cultured then induced with adipogenic agents for 3 days. The transcription level of miR-320-3p was examined by qRT-PCR. Stromal ST2 cells were transfected with miR-320-3p, followed by adipogenic treatment. Oil-red O staining and qRT-PCR were employed to assess the differentiation of adipocytes induced by miR-320-3p. Results The expression level of miR-320-3p increased in MSCs after adipogenic treatment (P < 0.01). Addition of miR-320-3p in ST2 cells promoted the formation of oil-red O positive adipocytes and up-regulated the expression levels of adipogenic transcription factors such as peroxisome proliferator-activated receptor γ (PPARγ), CCAAT/enhancer binding protein-α(C/EBPα) and the marker gene adipocyte fatty acid binding protein 4 (FABP4),compared to cells that transfected with miR-320-3p mimics (P<0.05). Conclusion miR-320-3p promotes ST2 cells differentiation into adipocytes.
