Regulatory of Jiaweisinisan on expression of hippocampal BDNF, NR1 and dental gyrus neurogenesis in rats with chronic stressed-depression
10.3969/j.issn.1001-1978.2016.04.025
- VernacularTitle:加味四逆散调控抑郁症大鼠海马BDNF、NR1表达及促进海马DG区神经再生的研究
- Author:
Can YAN
;
Yinwei LIU
;
Lili WU
;
Penghui ZHU
;
Yi PAN
- Publication Type:Journal Article
- Keywords:
depression;
hippocampus;
dental gyrus;
neurogenesis;
jiaweisinisan;
brain -derived neurotrophic factor;
N-methyl-D-aspartate receptor 1
- From:
Chinese Pharmacological Bulletin
2016;32(4):569-574
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the regulatory of Jiaweisini-san on expression of hippocampal BDNF, NR1 and dental gyrus ( DG ) neurogenesis in rats with chronic stressed-depression and its possible mechamisms. Methods Chronic unpredictable mild stress was used to establish the rat model of stressed depression. The expression of BrdU, NeuN, brain -derived neurotro-phic factor ( BDNF ) and N-methyl-D-aspartate recep-tor1 ( NR1 ) in hippocampal dental gyrus were detected by fluorescently labeled immunohistochemical method. In addition, BDNFmRNA was detected by in situ hy-bridization. Results Chronic stress could inhibit the proliferation of neural precursors in hippocampal DG ( P<0. 01 );the expression of BDNF decreased signifi-cantly in DG in model rats ( P <0. 01 ) , while the ex-pression of NR1 increased significantly ( P <0. 01 ) . JWSNS and Fluoxetine hydrochloride significantly en-hanced the amount of new proliferating cells and the number of neurons in unit area of DG ( P<0. 01 ) , in-creased the expression of BDNF ( P <0. 01 ) and de-creased the expression of NR1 in DG(P<0. 01). Con-clusion JWSNS could promote the neuronal prolifera-tion in hippocampal DG of rat with chronic stressed-de-pression,and may exert an effect of promoting the pro-liferation of neurons in hippocampal DG by enhancing the expression of BDNF and decreasing the expression of NR1 .
