FOXO3 expression in lung tissue and lung cancer stem cells and its implication

VernacularTitle:肺癌组织及其肺癌干细胞中FOXO3的表达及意义
Author: Zhiqiang ZHANG ; Yanrong YANG ; Yunxia LI
Publication Type:Journal Article
Keywords: lung neoplasms; FOXO3; cancer stem cells; RT-PCR; Western blot
From: Chinese Journal of Clinical and Experimental Pathology 2015;(8):880-884
CountryChina
Language:Chinese
Abstract: Purpose To detect the expression of FOXO3 in lung cancer specimens and lung cancer stem cell, for diagnosis and treat-ment of lung cancer. Methods Immunohistochemistry, semi-quantitative PCR and Western blot were used to detect FOXO3 protein expression in clinical specimens of lung cancer. PCR and Western blot verified FOXO3 mRNA and protein expression of lung cancer cells. Results Immunohistochemistry showed that the FOXO3 was significantly lower expressed in lung cancer tissues and the corre-sponding lung cancer stem cells;Detection of FOXO3 mRNA and protein by RT-PCR and Western blot showed both had significantly lower expression;as progression of the disease, there was a clear decreasing trend, in which negative expression rate of FOXO3 protein accounted for 66. 7% in all patients;at the same times, the rate of the expression gradually decreased with loss of differentiation and metastasis of the tumor. Conclusions FOXO3 shows low expression in lung tissue and lung cancer stem cells, while it is closely relat-ed to the degree of malignancy and lung cancer metastasis, which may be a new target for the treatment.