Effect of berberine on proliferation and apoptosis of MCF-7 cells
- VernacularTitle:黄连素对乳腺癌MCF-7细胞增殖和凋亡的影响
- Author:
Yajun ZHANG
;
Liting QIAN
;
Changjie CHEN
- Publication Type:Journal Article
- Keywords:
berberine;
MCF-7;
proliferation;
apoptosis;
JAK2/STAT3
- From:
Acta Universitatis Medicinalis Anhui
2015;50(9):1276-1280
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect and mechanisms of the Berberine on the human breast adenocarci-noma cell line MCF-7 . Methods MTT method was used to evaluate the proliferation effect of MCF-7 and the per-centage of apoptotic cells were determined by flow cytometric analysis. The expressions of JAK2, p-JAK2, p-STAT3, STAT3, Bax, Bcl-2, Cleavage-PARP and Cleavage-Caspase3 were detected by Western blotting. Results The results showed that BBR treatment decreased the activation of JAK2/STAT3 signal pathway and up-regulated the expression of Bax, Caspase3 and PARP activation with the decrease of the expression of Bcl-2. Wherefore, ex-pression of the constitutively active form of STAT3 could attenuate the effect of BBR on the MCF-7 cell. Conclusion Berberine can induces apoptosis and proliferation inhibition of breast adenocarcinoma cell lines of MCF-7 through inhibition of the JAK2/STAT3 signaling pathway.
