Anxiolytic effect of antianxietic compound prescription capsule on acute stress in rats and influence upon expression of ERK/CREB signaling pathway and BDNF in the brain of rats
10.3969/j.issn.1001-1978.2015.11.026
- VernacularTitle:复方抗焦虑胶囊对急性应激大鼠的抗焦虑作用及对大鼠脑内ERK/CREB信号通路和BDNF表达的影响
- Author:
Yuewei LYU
;
Jie LIU
;
Shuning SHI
;
Jianyou GUO
;
Yong LIU
;
Jinli SHI
- Publication Type:Journal Article
- Keywords:
antianxietic compound prescription cap-sule ( ACPC );
acute stress;
elevated plus maze ( EPM) test;
ERK/CREB signal pathway;
brain de-rived neurotrophic factor ( BDNF);
Western blot
- From:
Chinese Pharmacological Bulletin
2015;(11):1614-1619
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the pharmacodynamics of antianxietic compound prescription capsule ( ACPC ) on acute stress in rats and the influence upon the ex-pression of ERK/CREB signal pathway and brain-de-rived neurotrophic factor ( BDNF) in the cerebral cor-tex and hippocampus of rats. Methods The elevated plus maze ( EPM ) test was applied to observe the effects of ACPC on acute stress rats administered 7 d low-, medium- and high-dose ( 0. 75 , 1. 5 , 3 g · kg-1 ) . The expression of ERK/CREB signal pathway and BDNF in the cerebral cortex and hippocampus of rats were studied by using Western blot method. Re-sults In EPM, high-dose of ACPC increased signifi-cantly the rat open arm time ( OT%) ( P<0 . 05 ) and the percentage of open arm entries ( OE%) ( P <0. 05). In Western blot, the medium-dose of ACPC reduced significantly p-ERK1/2 expression in hippo-campus ( P <0. 05 ) , and high-dose group decreased significantly the expression of p-ERK1/2 and p-CREB in the cortex and hippocampus of rats ( P <0. 05 ) . High-dose group increased significantly the expression of BDNF in the cortex and hippocampus of rats ( P<0. 05 , P<0. 01 ) . Conclusion ACPC has anti-anxie-ty effect in the model of EPM, and its mechanism may be related to the ERK/CREB signal pathway and in-creased BDNF expression.
