Celastrol induces caspase-dependent apoptosis through ROS/JNK path-way in Saos-2 cells
10.3969/j.issn.1000-4718.2015.08.019
- VernacularTitle:雷公藤红素通过 ROS／JNK 途径诱导 Saos－2细胞发生 caspase 依赖的凋亡
- Author:
Zhiping HUANG
;
Lilong SHAO
;
Yangping RUAN
- Publication Type:Journal Article
- Keywords:
Celastrol;
Saos-2cells;
Apoptosis;
ROS/JNKpathway
- From:
Chinese Journal of Pathophysiology
2015;(8):1457-1461
- CountryChina
- Language:Chinese
-
Abstract:
AIM:ToexploretheeffectofcelastrolonapoptosisofSaos-2cellsanditsmechanism.METH-ODS:Saos-2 cells were treated with various concentrations of celastrol , and the cell viability was measured by MTT assay . Apoptosis and reactive oxygen species ( ROS) production were determined by flow cytometry .The protein levels of cleaved caspase-9, cleaved caspase-3 and phosphorylated JNK were evaluated by Western blot .RESULTS:The viability of Saos-2 cells was significantly inhibited by celastrol .Celastrol significantly induced apoptosis of Saos-2 cells.Celastrol signifi-cantly induced ROS production in the Saos-2 cells.Western blot analysis demonstrated that celastrol significantly increased the protein levels of cleaved caspase-9, cleaved caspase-3 and phosphorylated JNK in the Saos-2 cells.CONCLUSION:Celastrol induces caspase-dependent apoptosis through ROS/JNK pathway in Saos-2 cells.
