HPLC Simultaneous Determination of Vitexin and Isovitexin inMelastoma Dodecandrum

VernacularTitle:HPLC法测定地菍药材中牡荆素和异牡荆素的含量
Author: Dan CAO ; Yan JIANG ; Ruichao LIN ; Zhiqiang MA ; Jinfeng WANG ; Chongjun ZHAO
Publication Type:Journal Article
Keywords: M. dodecandrum; vitexin; isovitexin; content determination; HPLC
From: World Science and Technology-Modernization of Traditional Chinese Medicine 2015;(5):1012-1015
CountryChina
Language:Chinese
Abstract: This study was aimed to develop HPLC for determination of vitexin and isovitexin inM. dodecandrum. The HPLC column was SunFireTM C18 (4.6 mm× 150 mm, 5μm). The detection wavelength was 365 nm. The mobile phase was methanol-0.2% formic acid aqueous solution. The column temperature was 40℃. The flow rate was 1.0 mL·min-1. The results showed that the regression equations of vitexin and isovitexin wereY = 1× 106X– 14 396, Y = 1× 106X– 13 900, respectively. The linear ranges were 0.210μg - 1.050μg (r = 0.999) and 0.186μg - 0.930μg (r = 1.000), respectively. The recovery rates were 97.48% and 104.64%, respectively. The RSD were 2.32%and 1.51%, respectively. The sample contents of vitexin and isovitexin were 1.25 and 1.86 mg·g1, respectively. It was concluded that the method was simple, feasible and reproducible for the content determination of vitexin and isovitexin inM. dodecandrum.