Effects of proliferation and apoptosis of phloretin on human gastric cancer cells SGC-7901
- VernacularTitle:根皮素对胃癌细胞SGC－7901凋亡的影响及可能机制
- Author:
Hui WANG
;
Handong WU
;
Zheng LIU
- Publication Type:Journal Article
- Keywords:
phloretin;
gastric cancer cell;
apoptosis;
proliferation
- From:
Chinese Journal of Biochemical Pharmaceutics
2015;(8):34-37
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the proliferative and apoptotic effects of phloretin on gastric cancer cell andthe possible mechanisms. Methods SGC-7901 were treated with different concentrations of phloretin(40,80,160 mg/L), and the cell morphological alterations were detected by using Hoechst33258 staining, cell activity were detected by MTT assay, cell apoptosis, cell cycle progression, mitochondrial trans-membrane potential and intracellular calcium homeostasis were detected by flow cytometry.ResuIts After treated with different concentrations of phloretin at different times, SGC-7901 cell showed morphological alterations.Phloretin could inhibite the proliferation of SGC-7901 cell line, and induced its apoptosis in a dosage and duration dependent manner.Cell cycle was arrested at G1 phase, mitochondrial trans-membrane potential dropped, intracellular free Ca2 +increased.ConcIusion phloretin can induce apoptosis of SGC-7901 via arresting cell cycle progression, reducing mitochondrial trans-membrane potential and disturbing intracellular calcium homeostasis.
