Expression of Rous Sarcoma Virus Enhancer Factor Gene in Human Hepatocellular Carcinoma.
- Author:
Boo Ahn SHIN
1
;
Kyung Kun KIM
;
Chol Kyoon CHO
;
Hyun Chul LEE
;
Jong Suk OH
;
Joon Haeng RHEE
;
Mee Young CHANG
Author Information
1. College of Pharmacy, Medical School, Chonnam National University, Kwangju 500-757, Korea.
- Publication Type:Original Article
- Keywords:
RSV;
LTRs;
Hepatocellular carcinoma;
Enhancer factor;
Y-box
- MeSH:
Animals;
Blotting, Northern;
Carcinoma, Hepatocellular*;
Cell Line;
Clone Cells;
DNA, Complementary;
Gene Library;
Genomic Library;
Hep G2 Cells;
Humans*;
Liver;
Rats;
RNA, Messenger;
Rous sarcoma virus*;
Sarcoma, Avian*;
Sequence Analysis;
Terminal Repeat Sequences
- From:Journal of the Korean Cancer Association
1997;29(4):547-554
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: We have previously cloned three enhancer factor genes encoding proteins that bind to long terminal repeats (LTRs) of Rous sarcoma virus. Among these genes, RSV- EF-I gene is expressed in rat hepatoma tissues and several proliferating cell lines but not in normal rat liver tissues. We have isolated the human homologue of RSV-EF-I gene and examined its expression in human hepatocellular carcinoma tissues. MATERIALS AND METHODS: We have screened the human genomic library and cDNA library of Hep G2 cell line derived from human hepatocellular carcinoma to isolate the human homologue of RSV-EF-I gene. RESULTS: We have isolated one cDNA clone containing about 1.5 kb insert and sequenced. Sequence analysis reveals that this human homologue of RSV-EF-I gene has a high similarities to human YB-1 mRNA, human DNA-binding protein B (dbpB) gene and other Y-box protein genes. It is expressed in human hepatocellular carcinoma but very slightly in normal human liver tissues in Northern blot analysis. CONCLUSION: Our data suggest that the human homologue of RSV-EF-I gene presumably belongs to Y-box protein family genes and plays a role in the transformation of the human hepatoma cells.
