The Study of miR-204 Regulates BDNF/TrkB Expression in Epileptic Neurons
10.3969/j.issn.0253-9896.2014.03.007
- VernacularTitle:miR-204对癫痫神经元中BDNF/TrkB信号通路的影响
- Author:
Wei CHANG
;
Liping PAN
;
Qiujing WU
;
Yijun SONG
- Publication Type:Journal Article
- Keywords:
epilepsy;
hippocampus;
neurons;
receptor,trkB;
microRNAs;
brain-derived neurotrophic factor;
miR-204
- From:
Tianjin Medical Journal
2014;(3):214-216
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of miR-204 on BDNF/TrkB signaling and pathogenesis on the neuron model of epilepsy. Methods Primary hippocampal neurons were cultured in vitro for 7 days, and were divided into control group, control+BDNF group, epilepsy group, epilepsy+BDNF group , control+miR204 group, epilepsy+miR204 group and ep-ilepsy+miR204+BDNF group. The epilepsy model of hippocampal neurons was established by being exposed to Mg2+free me-dia for 3 hours. The miR-204 lentivirus vector was constructed. The effect of miR-204 on BDNF/TrkB expression was detect-ed by immunohistochemistry, patch clamp and Western blot technique. Results Compared with the control group, the TrkB phosphorylation level was higher in control+BDNF group. The TrkB phosphorylation level was lower in epilepsy+BDNF group than that of control+BDNF group, but it was higher than that of epilepsy group. The TrkB phosphorylation level was higher in epilepsy+miR204+BDNF group than that of epilepsy+BDNF group and epilepsy+ miR204 group. Conclusion BDNF and miR-204 can improve the inhibitory condition of BDNF/TrkB signaling and may play an important role in alleviat-ing epilepsy disease.
