Effect of dimethylaminoethano and compound amino acid on collagens Ⅰ and Ⅲ mRNA expression in the skin of D-galactose induced aging model in rats
10.3760/cma.j.issn.1671-0290.2014.06.016
- VernacularTitle:二甲氨基乙醇和复方氨基酸溶液对衰老大鼠皮肤Ⅰ型和Ⅲ型胶原蛋白mRNA表达的影响
- Author:
Su LIU
;
Ying SUN
;
Cailing ZHAO
;
Dalie LIU
- Publication Type:Journal Article
- Keywords:
Mesotherapy;
Dimethylaminoethanol;
Compound amino acid;
Anti-aging;
Ⅰ and Ⅲ collagen;
PCNA;
D-galactose
- From:
Chinese Journal of Medical Aesthetics and Cosmetology
2014;20(6):455-459
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of dimethylaminoethano (DMAE) and compound amino acid injection (AA) by mesotherapy on collagen Ⅰ and collagen Ⅲ mRNA expression levels of D-galactose-induced chemical skin aging rats.Methods 80 rats were randomly divided into individual experimental group.At 18 days after D-gal induction,rats of aging treatment groups were treated with intradermal microinjection of 0.2％ DMAE+AA,0.1％ DMAE+AA,0.2％ DMAE,0.1％ DMAE,AA,and saline,respectively,once a week for 4 weeks.At 42 days after treatment,the skin wounds were harvested.HE,PCNA,hydroxyproline and collagen Ⅰ and collagen Ⅲ mRNA expression levels of every group skins were detected.Results Dermal thickness,hydroxyproline content and collagen type Ⅰ and Ⅲ mRNA expression levels of 0.1％ DMAE+ AA and 0.2 ％ DMAE+ AA groups were significantly improved (P＜0.05),but PCNA expression of sham control group was significantly higher than all of aging groups (P＞0.05).Conclusions Local co-injection of DMAE and compound AA directly into targeted tissue under mesotherapy has marked anti-aging effects on D-galactose induced skin aging model of rat by increasing the dermal thickness,collagen content and acceleration of collagen synthesis.
